GHK-Cu (50MG)

GHK-Cu — the Cu(II)-coordinated complex of the tripeptide glycyl-L-histidyl-L-lysine — supplied by BioFusion as a vacuum-sealed lyophilized vial of 50mg, vacuum-stoppered and amber-shielded for copper-peptide laboratory protocols.

Coordination chemistry

GHK-Cu is one of the most-cited copper-coordination complexes in peptide research. The Cu(II) ion sits in a square-planar geometry with three coordination bonds to the histidine imidazole nitrogen, the deprotonated amide nitrogen of the Gly-His peptide bond, and the N-terminal glycine amine, plus a fourth coordination site that varies with solvent. The free GHK-Cu chemistry primer walks through binding geometry, Fmoc synthesis, and stability considerations in detail.

Synthesis & verification

BioFusion’s GHK-Cu reference is produced by solid-phase Fmoc-SPPS synthesis of the Gly-His-Lys tripeptide, followed by stoichiometric loading of Cu(II) and post-coordination purification. Each released lot is verified for identity (LC-MS), peptide purity (reverse-phase HPLC-UV ≥99.0%), copper content (ion chromatography), and moisture (Karl Fischer). The signed Certificate of Analysis ships with every order; the COA library retains all historical lot documentation.

Storage & handling

Lyophilized GHK-Cu remains stable at −20 °C for the printed shelf life. After reconstitution, the complex is photolabile — store in amber vials at 2-8 °C and use within fourteen days. The blue color of the dissolved complex (λmax ≈ 525 nm) is diagnostic of intact Cu(II) coordination; pronounced fading suggests reduction or dissociation. The reconstitution calculator handles working-concentration math.

Why BioFusion

Copper-peptide complexes synthesized by suppliers without ion-chromatography stoichiometry checks frequently ship under-loaded, over-loaded, or oxidized — meaning the labeled “GHK-Cu” is actually a mixture of free GHK and CuCl₂. BioFusion verifies copper stoichiometry on every lot and publishes the result on the batch transparency ledger. Bulk discounts trigger at three+ vials; members save 25% on every order plus complimentary U.S. shipping for 365 days.

For research and laboratory use only. This product is not a drug, food, or cosmetic, and is not intended for diagnostic, therapeutic, or in vivo human use.